Maximize your quantitative imaging with the new FirstLight UV Illuminator! Unique design provides extremely uniform UV illumination, which is critical for accurate quantitative analysis.
The FirstLight UV Illuminator represents a unique highly uniform excitation source for quantitative fluorescent imaging in a wide range of genomic and proteomic applications:
- Electrophoretic separation and quantiation of 1D and 2D protein separations
- Solid phase immunoassay
- DNA quantitation
- RNA quantitation
- Application Note describes use of the FirstLight UV Illumination instrument for use in quantitative DNA and protein analysis.
The FirstLight™ UV Illuminator unique patented design emits 302 nm ultraviolet excitation and combines a specially designed, high density grid array ultraviolet lighting configuration with a phosphor coating to generate exceptionally uniform ultraviolet illumination. It produces less than 5% coefficient of variance (CV) across the full imaging surface, which is essential for capturing high quality images for documentation and quantitative analysis. The design assures consistent sensitivity and dynamic range for achieving accurate and reproducible DNA, RNA and protein gel analysis results. No matter where the gel is placed on the optical band pass filter surface, uniformity of ultraviolet illumination is assured for direct gel-to-gel comparison.
Main Features
- High sensitivity and dynamic range are consistent across the illumination surface
- Software correction of uniformity is no longer required
- Accurate gel to gel comparison
- Uniformity now supplies <5% coefficient of variance (CV) across the full filter area
- When used with UVP’s BioImaging System and CCD camera, the configurations provides a low cost solution compared to laser scanning
- Construction design includes an electronic ballast for increased stability and reduced power consumption
- Compact size
- Illuminator includes a Gel-Cutter, Focus Target and UV Blocking Cover
