SmartGlow DNA Stains: Safe and Efficient Nucleic Acid Visualization
BLACK FRIDAY BOGO MIX AND MATCH SPECIAL PROMOTION! Purchase one tube of SmartGlow stain and receive your choice of a SmartGlow Loading Dye or Pre-Stain FREE! Use promo code: TURKEY24SG. Offer expires December 6,2024. IMPORTANT: PLEASE ADD THE SECOND SMARTGLOW STAIN TO YOUR CART IN ORDER TO APPLY THE PROMO CODE.
SmartGlow DNA stains offer a safer alternative for nucleic acid visualization in gel electrophoresis. These innovative stains are designed for optimal excitation of DNA and RNA using both UV and visible blue light transilluminators.
Key Features of SmartGlow DNA Stains:
- Non-mutagenic formula
- Easy disposal without special methods
- Compatible with UV and blue light excitation
- Suitable for dsDNA, ssDNA, and RNA visualization
SmartGlow PS (Pre Stain) can be used as a direct replacement for Ethidium Bromide in agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to dsDNA, ssDNA and RNA. SmartGlow PS exhibits excitation peaks at 290nm and 490nm, allowing it to be used with UV and blue light.
SmartGlow™ LD (Loading Dye) is supplied at 6X concentration and is added directly to the samples. No dye needs to be added to the gel or running buffer. After electrophoretic separation, view and document your results using a UV or blue light illuminator. SmartGlow™ LD emits green fluorescence when bound to dsDNA, ssDNA and RNA.
Protocol for SmartGlow PS
- Prepare 100ml of agarose or polyacrylamide solution.
- Add 5ul of SmartGlow PS stain to the gel solution before pouring gels.
- For enhanced results, add SmartGlow PS to the running buffer at a ratio of 2.5ul to 5ul per 100ml. Adding SmartGlow PS to the running buffer will result in increased sensitivity and better detection of small quantities of nucleic acid.
- After electrophoresis is complete, view the gel using a UV or blue light illuminator.
Protocol for SmartGlow LD
- Prepare agarose for polyacrylamide solution.
- Pour gels.
- Mix SmartGlow LD with samples and DNA markers at a 1:5 (dye to sample) dilution.
- After electrophoresis is complete, view the gel using a UV or blue light illuminator
Documentation
SmartGlow FAQs here
SmartGlow Pre-Stain instruction manual here
SmartGlow Loading Dye instruction manual here
Quantity: 1mL
