Trypan Blue Stain 0.4% – Cell Viability & Cell Counting Reagent
Trypan Blue Stain 0.4% is a vital dye used to assess cell viability by distinguishing viable from non-viable cells. Viable cells remain unstained (colorless), while nonviable cells take up the blue dye. This product is ideal for cell counting applications using LUNA brightfield counters.
T13001 - Trypan Blue Stain 0.4% 2 x 1mL/200 tests
Features & Benefits
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Standard 0.4% concentration, widely used in cell culture protocols
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Available in non-sterile and sterile-filtered versions for sensitive applications
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Compatible with LUNA™ II, III, FL, BX7 and FX7 automated cell counters
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Accurate, reliable viability assessment for research, diagnostics, or bioprocessing
How To Use
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Mix equal volumes of cell suspension with Trypan Blue Stain (0.4%)
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Incubate at room temperature for 1-2 minutes
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Load into the cell counter / hemocytometer
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Count viable vs nonviable cells based on membrane integrity
Note: Delay beyond 5 minutes can affect accuracy as viable cells may begin to uptake dye.
Specifications
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Molecular formula: C₃₄H₂₈N₆O₁₄S₄
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Molecular weight: 872.88 g/mol
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Appearance: Dark blue liquid
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Storage: Room temperature (protect from light)
Ordering information
| Cat # |
Description |
Qty |
| T13001 |
Trypan Blue Stain 0.4%, non-sterile |
2 × 1.0 mL |
| T13011 |
Trypan Blue Stain 0.4%, sterile-filtered |
2 × 1.0 mL |
FAQ
Why use 0.4% concentration?
The 0.4% Trypan Blue is standard for viability tests. Lower concentration may under-stain non-viable cells; higher concentration may increase background.
Can I use this with LUNA™ Brightfield counters?
Yes. It is fully compatible with the LUNA™ II, III, FL, BX7 and FX7 counters.
How long can the dye be stored?
Refer to the MSDS / CoA for details. Once opened, avoid contamination; sterile-filtered version helps in such cases.
