Available in 2.5 mL bulk quantities!
FluoroVue™ Nucleic Acid Gel Stain (10,000X) is specially designed for in-gel use and is a safer replacement for conventional Ethidium bromide (EtBr), which poses a significant health and safety hazard for its user. It is a fluorescent stain which offers high sensitivity detection of double-stranded or single-stranded DNA and RNA in a convenient manner. The FluoroVue™ Nucleic Acid Gel Stain offers high sensitivity (Table 1 and Fig. 2) that is several times greater than EtBr.
Different staining methods for using the FluoroVue™ Nucleic Acid Gel Stain
|In-gel staining||4ul||0.14ng||very good|
|Staining during electrophoresis||30ul||0.56ng||very good|
For detailed protocols of different staining methods: please see pages 5~7. We recommend using an in-gel staining method for optimal effect. 1 With a mini horizontal gel electrophoresis system: Combine 40 ml of agarose gel with 300 ml running buffer. The regular post staining buffer volume is 100 ml. 2 Sensitivity is evaluated according to the 4 kb band of DM3100
The emission and exitation spectrum of FluoroVue™ Nucleic Acid Gel Stain (NS1000)
FluoroVue™ Nucleic Acid Gel Stain shows a green-yellow fluorescence under blue light excitation. The sensitivity of NS1000 is about 0.14 ng (arrow) for a 4 kb fragment.
Proprietary dye in a 10,000X concentration.
Protected from light:
4°C for 12 months
-20°C for 24 months
Dispose of the stain in accordance to local rules and regulations. The fluorescent staining dye stock solution should be handled with particular caution because the solvent is known to facilitate the entry of organic molecules into tissues. There is no data that addresses the mutagenicity or toxicity of the fluorescent dye in humans. However, the fluorescent dye binds to nucleic acids, thus it should be recognized as a potential mutagen and used with appropriate care.