FluoroVue™ Nucleic Acid Gel Stain (10,000X) | Nucleic Acid Dye

FluoroVue™ Nucleic Acid Gel Stain (10,000X)

Available in 2.5 mL bulk quantities!

FluoroStain DNA Fluorescent Staining Dye (Green, 10,000×)

 

FluoroVue™ Nucleic Acid Gel Stain (10,000X) is specially designed for in-gel use and is a safer replacement for conventional Ethidium bromide (EtBr), which poses a significant health and safety hazard for its user. It is a fluorescent stain which offers high sensitivity detection of double-stranded or single-stranded DNA and RNA in a convenient manner. The FluoroVue™ Nucleic Acid Gel Stain offers high sensitivity (Table 1 and Fig. 2) that is several times greater than EtBr.

Features

  • Excellent for in-gel staining 
  • Sensitivity: 0.14 ng (DNA) or 1 ng (total RNA) 
  • A safer alternative to EtBr 
  • Compatibility: suitable to blue or UV light 
  • Increased cloning efficiency (blue light) 

    Different staining methods for using the FluoroVue™ Nucleic Acid Gel Stain

    Required Dye Sensitivity  Convenience
    In-gel staining 4ul 0.14ng very good
    Staining during electrophoresis 30ul 0.56ng very good
    Post-stain 10ul 0.56 good

     

    For detailed protocols of different staining methods: please see pages 5~7. We recommend using an in-gel staining method for optimal effect. 1 With a mini horizontal gel electrophoresis system: Combine 40 ml of agarose gel with 300 ml running buffer. The regular post staining buffer volume is 100 ml. 2 Sensitivity is evaluated according to the 4 kb band of DM3100 

    The emission and exitation spectrum of FluoroVue™ Nucleic Acid Gel Stain (NS1000)

    FluoroVue™ Nucleic Acid Gel Stain shows a green-yellow fluorescence under blue light excitation. The sensitivity of NS1000 is about 0.14 ng (arrow) for a 4 kb fragment.

    Contents

    Proprietary dye in a 10,000X concentration.

    Storage

    Protected from light:
    4°C for 12 months
    -20°C for 24 months

    Download MSDS

    Caution

    Dispose of the stain in accordance to local rules and regulations. The fluorescent staining dye stock solution should be handled with particular caution because the solvent is known to facilitate the entry of organic molecules into tissues. There is no data that addresses the mutagenicity or toxicity of the fluorescent dye in humans. However, the fluorescent dye binds to nucleic acids, thus it should be recognized as a potential mutagen and used with appropriate care.

    Ordering information:

    • Catalog number: NEB-NS1000 (Qty - 500uL)/NEB-NS1001 (Qty - 5 x 500uL)
    • Country of origin: Taiwan
    • Ships from: Canada
    • Typically ships with 48 hours upon receipt of an order

     

    On sale



    Vendor SMOBio
    Type Nucleic Acid Stain
    Collections Consumables
    Tags SMOBIO |

    Have a question about this product?