FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000×) | Nucleic A

FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000×)

Ultra-high sensitivity! Detect down to 0.04 ng DNA. Available in 2.5mL bulk quantities!

FluoroStain DNA Fluorescent Staining Dye (Green, 10,000×)

 

FluoroStain™ DNA Fluorescent Staining Dye is designed to be a safer replacement for conventional Ethidium bromide (EtBr) which poses a significant health and safety hazard for its users. The FluoroStain™ DNA Fluorescent Staining Dye offers at least 10 times sensitivity in DNA detection levels, and is capable of detecting double stranded DNA (dsDNA) fragments up to 0.04 ng in electrophoresis analysis (Fig. 1). The FluoroStain™ DNA Fluorescent Staining Dye shows a high specificity to the dsDNA, with negligible background signal, making the destaining process entirely optional. FluoroStain™ DNA Fluorescent Staining Dye is compatible with both the conventional ultra violet gel-illuminating systems as well as the less harmful long wave length blue light illumination systems. The emission when bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm

Features

  • Excellent for post staining
  • Sensitivity: 0.04 ng DNA
  • A safe alternative to EtBr
  • Compatibility: suitable for blue or UV light
  • Increased cloning efficiency (blue light)

DS1000 FluoroStain DNA Fluorescent Staining Dye (Green, 10,000×), emission as bound to dsDNA is 522 nm while its excitation peaks are at 270, 370 and 497 nm.

DS1000 FluoroStain Ames Test

SMOBIO DS1000 DNA staining dye shows no mutagenic activity in the Ames test.
Mutagenic activity of DNA staining dyes SYBR Green I (Invitrogen), DS1000 (SMOBIO) and Ethidium Bromide (EtBr) were evaluated by Ames test. The DNA staining Dyes were diluted to their respective working concentration: SYBR Green I: 10000X dilutions; Ethidium Bromide (10 mg/ml): 10000X dilutions; DS1000: 10000X dilution. When treated with DS1000, the frame-shift mutation-indicating tester strain TA98 showed no increases in revertant frequency when compared to H2O and DMSO (solvents for both SYBR Green I and DS1000), while Ethidium Bromide induced a statistically significant increase in the number of revertants. All tests were performed using the plate-incorporation method of exposure as described by Maron and Ames. Compounds were tested using Salmonella typhimurium tester strain TA98 with the rat-liver S9 homogenate.

Contents

FluoroDye DNA Fluorescent Loading Dye is stored in 6× concentration in 60% glycerol and buffered with Tris-HCl and EDTA, containing Bromophenol blue, Xylene cyanol FF and Orange G as tracking dyes.

Storage

Protected from light:
4°C for 12 months
-20°C for 24 months

Download MSDS

Protocols:

1. Perform agarose gel electrophoresis as usual according to your standard protocol.
2. Dilute FluoroStain™ DNA Fluorescent Staining Dye reagent 10,000 folds in a TE, TAE, or TBE buffer.

  • Buffered solutions increase the stability of fluorescent staining dye. When diluted in water, it should be used within 24 hrs.
  • Use a plastic container. Glass containers are not recommended, as they absorb much of the dye in staining solution.
  • Protect the staining container from light by covering it with aluminium foil, or place it in the dark.
  • The staining solution may be stored in the dark at room temperature for up to one week.
3.Immerse the gel in a staining solution (1X) and incubate at room temperature for 10 - 30 minutes.
  • Staining time varies with the thickness of the gel and percentage of agarose. If needed, agitate the gel gently at room temperature to shorten staining time.
  • No de-staining required.

4. Visualize or photograph the gel with UV or bluelight illumination (blue-light is recommended).

It is important to clean the surface of the illuminator before and after each use with deionized water. Otherwise, fluorescent dyes will accumulate on the surface and create a high fluorescent background.

Video cameras and CCD cameras have a different spectral response compared to the black-and-white print film and therefore may not exhibit the same sensitivity.

Ordering information:

  • Catalog number: NEB-DS1000 (Qty - 500uL)/NEB-DS1001 (Qty - 5 x 500uL)
  • Country of origin: Taiwan
  • Ships from: Canada
  • Typically ships with 48 hours upon receipt of an order

 

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Vendor SMOBio
Type Nucleic Acid Stain
Collections Consumables
Tags SMOBIO |

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