Dectect down to .14ng DNA. Available in 5mL bulk quantities!
FluoroDye DNA Fluorescent Loading Dye is a ready-to-use 6× DNA loading dye designed for fast qualitative electrophoresis analysis. Containing sensitive fluorescent dye with high specific affinity towards double stranded DNA (dsDNA), the FluoroDye Fluorescent DNA Loading Dye has negligible background and renders destaining process unnecessary. The FluoroDye DNA Fluorescent Loading Dye allows the user to immediately visualise electrophoresis result upon completion or to monitor the electrophoresis in real time. FluoroDye DNA Fluorescent Loading Dye is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system. FluoroDye emission as bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm
FluoroDye DNA Fluorescent Loading Dye is stored in 6× concentration in 60% glycerol and buffered with Tris-HCl and EDTA, containing Bromophenol blue, Xylene cyanol FF and Orange G as tracking dyes.
Protected from light
-20°C for 24 months
1. Mix FluoroDye™ DNA Fluorescent Loading Dye with the DNA sample at a volume ratio of 1:5.
2. Mix FluoroDye™ and DNA sample thoroughly. A brief incubation time (1~2 minutes) for proper interaction between FluoroDye™ and DNA sample is highly
3. Perform agarose gel electrophoresis (avoid light).
4. Visualize or photograph the gel with UV or bluelight illumination (blue-light is recommended).
It is important to clean the surface of the illuminator before and after each use with deionized water. Otherwise, fluorescent dyes will accumulate on the surface and create a high fluorescent background.
Video cameras and CCD cameras have a different spectral response compared to the black-and-white print film and therefore may not exhibit the same sensitivity.